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KMID : 0360220140550081202
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Journal of the Korean Ophthalmological Society
2014 Volume.55 No. 8 p.1202 ~ p.1207
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Steroid-Induced Ocular Hypertension Model in the Mice
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Kim You-Ra
Kang Wan-Seok
Kweon Eui-Yong
Cho Nam-Chun
Lee Dong-Wook
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Abstract
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Purpose: To determine whether rat eyes develop increases in intraocular pressure (IOP) in response to a topically applied corticosteroid and to investigate the relationship between ocular hypertension and apoptosis of retinal ganglion cells.
Methods: IOP was monitored by rebound tonometry in a group of 10 rats that received topically administered dexamethasone in both eyes (experimental) and in another group of 5 rats that received artificial tears (control) three times daily for 4 weeks after the establishment of baseline IOP values. Only eyes that increased by more than 50% compared with the basal IOP were administered once per day for 5 weeks. After 8 weeks, selective immunofluorescence stain for retinal ganglion cells and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) stain were conducted.
Results: Among 20 experimental eyes, 11 eyes (55%) showed a greater than 50% increase in IOP compared with basal IOP. After 8 weeks, the mean IOPs for the experimental and control groups were 11.8 ± 1.4 mm Hg and 18.5 ± 1.0 mm Hg, respectively (p < 0.01). The counts of central retinal ganglion cells (RGCs) were 2718 ± 240 and 2612 ± 443, respectively (p = 0.294). The results of the TUNEL stain also showed no differences.
Conclusions: Rat eyes exhibit a steroid-induced ocular hypertensive response with no local complications. However, maintaining ocular hypertension increased by 50% for two months was not enough to detect changes in RGCs.
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KEYWORD
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Ocular hypertension, Retinal ganglion cell, Steroid-induced glaucoma
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